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Biosurfactants are therefore the natural choice for such processes as they possess a lot of advantages over synthetic surfactants, such as lower toxicity, biodegradability, and effectiveness at a wide range of p H and temperature values [6, 7].
Bacterial biosurfactants were initially proposed to function as emulsifiers of biodegradable hydrocarbons .
while using sugar cane molasses or glycerol as substrates.
The biosurfactant-producing strains Lactobacillus cellobiosus TM1, Lactobacillus delbrueckii N2, and Lactobacillus plantarum G88 were isolated and identified in previous works [16, 25].
Despite the numerous advantages of lactobacilli biosurfactants, they are less effective in reducing surface tension of water (approximately 36–40 m N/m) compared to other biosurfactants which are able to reach values lower than 30 m N/m.
Moreover, they are not yet used intensively for industrial productions, since expensive substrates are required for their production and they present relatively low productivities (20–100 g/L) [13, 20, 27], which hampers their widespread use and commercialization [5, 28, 29].
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The potential of three indigenous bacterial strains (Lactobacillus delbrueckii N2, Lactobacillus cellobiosus TM1, and Lactobacillus plantarum G88) for the production of biosurfactants using sugar cane molasses or glycerol as substrates was investigated through emulsifying, surface tension, and antimicrobial activities.